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Vol. 11 · Issue 17 · 14 March 2026 Peer-reviewed husbandry, field-tested
Vol. 11 · Issue 17 · 14 March 2026 EN · °F
IguanaNaut

The reptile-keeping journal for keepers who treat husbandry like a science.

A weekly herpetoculture publication turning peer-reviewed research into field-tested protocols — every protocol run by three independent keepers for ninety days before it runs here.

Protocol of the Week

Calibrating UVB gradients for leopard geckos (Eublepharis macularius) in 40-gallon bioactive enclosures

Read the full protocol →
A leopard gecko resting under a cork bark hide in a 40-gallon bioactive enclosure, photographed in soft window light.
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Protocol of the Week · Vol. 11 Issue 17

Calibrating UVB gradients for leopard geckos (Eublepharis macularius) in 40-gallon bioactive enclosures

The case for UVB in crepuscular geckos has moved from contested to consensus in the last six years. What remains contested is dose: how much, where, and how to measure it in an enclosure whose canopy is half live plant. This protocol walks through a three-zone gradient, the meter that actually reads it, and the four-week adjustment cycle that three independent keepers used to dial in basking behavior without elevating D3-supplementation risk.

Why this protocol exists

Two of the three keepers in our review cycle had leopard geckos that spent the entire photophase under the cool-side hide. The third had the opposite problem — chronic surface basking, low ambient humidity, and shed retention around the toes. Same species, same fixture manufacturer, three different outcomes. The variable wasn't the lamp; it was the measurement.

What you'll measure

A UVB gradient isn't a number — it's three numbers across one enclosure. The cool-side ambient should sit between 5–15 µW/cm² (roughly UVI 0.5–1.0), the mid-zone basking ledge at 25–40 µW/cm² (UVI 2.0–3.0), and the far basking surface at no more than 50 µW/cm² (UVI 4.0). Above UVI 4.0 in this species, keepers in our review cycle began to see photo-avoidance and reduced surface activity within ten days.

The meter matters. We recommend a Solarmeter 6.5R for keepers who need a numeric reading, and a printed UV index card as a sanity check during weekly maintenance. Both are used in the field-test protocol; both are referenced with model and calibration date in the Appendix.

The 90-day field log

Each of the three keepers logged basking duration, shed quality, fecal consistency, and ambient humidity at the same three points in the enclosure, every Monday and Thursday, for ninety days. We pulled the aggregate into the appendix; keepers who want to replicate the protocol can fork the field log and adapt the species target columns.

Primary sources: Baines et al. (2016), Journal of Herpetological Medicine & Surgery 26(3); personally communicated data from the IguanaNaut Mortality & Morbidity Database, case cohort M-2025-LEO-114.

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Every protocol we publish maps to one of these three systems. Pick the system you're refining this week; the latest peer-reviewed work in each is below.

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The M&M Database

The field's living necropsy record.

11,432 anonymized incident logs contributed by 2,100+ verified keepers across nine years. Each entry logs husbandry parameters, presenting condition, veterinary findings where available, and outcome. Used by 60+ exotics veterinary practices across the US, UK, and EU as a baseline reference for captive-incident pattern recognition.

11,432 anonymized incident logs
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60+ exotics practices using it
92 countries represented

We accept contributions from verified keepers only — submission requires a one-time husbandry attestation and a photo of the enclosure parameters in question. Entries are reviewed by an advisory DVM before publication.

Climate & Lighting Calculator

Size your UVB, basking, and humidity to the exact enclosure — not to the lamp box.

A custom tool used by 22,000+ keepers to specify lamp wattage, fixture distance, basking zone dimensions, and humidity envelope from your enclosure's length, width, height, and target species. No account, no email, no tracking.

Recent field-test

"Used it to size a 4×2×2 for a juvenile Varanus exanthematicus. The basking zone recommendation matched what I'd worked out by hand — and the humidity envelope caught a problem I'd missed."

— Verified keeper, contributor #2,041
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Species care sheet index

Browse by species. Every care sheet is peer-reviewed and field-tested.

Sortable. Filter by husbandry system, citation tier, or last-review date. Currently indexed: 240 species across lizards, snakes, chelonians, and amphibians.

Species Common name Last review Tier Systems covered
Eublepharis macularius Leopard gecko 14 Mar 2026 Peer-reviewed 3 of 3 Open →
Pogona vitticeps Bearded dragon 07 Mar 2026 Peer-reviewed 3 of 3 Open →
Python regius Ball python 28 Feb 2026 Breeder-log 3 of 3 Open →
Varanus exanthematicus Savannah monitor 21 Feb 2026 Editorial 2 of 3 Open →
Stigmochelys pardalis Leopard tortoise 14 Feb 2026 Peer-reviewed 3 of 3 Open →
Dendrobates tinctorius Dyeing poison frog 07 Feb 2026 Breeder-log 2 of 3 Open →
Pantherophis guttatus Corn snake 31 Jan 2026 Peer-reviewed 3 of 3 Open →

All 240 species care sheets are translated into 7 languages through a partnership with the European Herpetological Society. Browse the full index →

Editorial advisory board

Nine contributors. Three DVMs. One tenured herpetologist.

Every protocol we publish has been read by at least one member of this board. Every clinical claim has been read by at least one of our three reptile-medicine DVMs. Names are listed by board role; individual veterinary partners are not named without written consent.

3 DVMs specializing in reptile medicine Review every clinical claim before publication
1 Tenured herpetologist Reviews taxonomy, natural-history framing, and primary-literature citations
5 Breeders & keepers AZA-affiliated and private — run the 90-day field-test cycle
6 Full-time editors IguanaNaut Media, LLC · Austin, TX · core team
Press & citations

Where IguanaNaut has been read, recommended, and cited.

2024

Featured in BioReddit's Top 25 Herpetology Resources for the third consecutive year.

2023

Cited in the Journal of Herpetological Medicine, Vol. 31, Issue 2, as a non-academic reference.

2023

Featured in BioReddit's Top 25 Herpetology Resources.

2022

Featured in BioReddit's Top 25 Herpetology Resources.

Ongoing

Recommended resource by 60+ exotics veterinary practices across the US, UK, and EU.

2014

Founded in March 2014 by two AZA-affiliated keepers and a published herpetologist. 1,840+ peer-reviewed care articles and long-form protocols since launch.

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